Growth stage dependent-detection of maize E3-type ubiquitin ligase gene expression

─One of the main pathways of post-translational regulation of the levels of specific proteins in eukaryotic cells is based on ubiquitination process in which E3 ligases such as SINAT5 transfer the ubiquitin to the target proteins. For further identification, the expression and the relative level maize SINAT5 transcript were analyzed using reverse transcription and polymerase chain reaction (RT-PCR) with template leaf materials collected separately from the vegetative and the flowering stages of maize plants grown under the normal growth conditions. The obtained results not only confirmed the presence of SINAT5 transcript in the leaf tissues of both growth stages but also revealed its abundance in the leaf samples of flowering stage that may be in agreement with its large number of targets for ubiquitination in flowering stage of test plant.